hate to make generalizations when spotlighting predators, but as was mentioned earlier, predator eyes and prey eyes are located in the skull for a reason. But that said, the height of the eyes above ground is also something to look for. A deers almost crystal white reflection 3-1/2 to 4 ft off the ground vs a yote 18-20" off the ground usually delineates species differences without color concerns. Deer will "blink" at you also whereas a yote often turns its head and one eye remains lit up. Nothing other than body ID should be the go-ahead for putting a shot downrange while hunting at night. SOMETIMES (?) coyote eyes will put off a slight green/emerald tint compared to an almost pure white crystal reflection from a deer,horse,cow. Other colors at night, mice/rats/bunnies = pink, 3 bright reflections in a triangle pattern = train. Good luck night hunting.