Luke
The longer you pause, the more chance the animal has of becoming distracted with something else. Electronic calls basically have no pauses and work well. The good thing about hand calls is you can put your own "spin" on how you call and sound different than other callers.
There are about 100 different things that are more important when calling predators than the timing of the call sequence. Success is going to be determined by the other variables in your hunt.
Doc
